FBQ1: The group of enzymes that cleave without the addition of water is known as ______ Answer: *Lyases* FBQ2: Low molecular weight organic substances which enzymes could require for their catalytic activities are known as ____ Answer: *coenzymes* FBQ3: The enzyme substrate theory in which the enzymes undergoes a conformation change to which the substrate binds is known as ____________ theory Answer: *induced fit* FBQ4: The pH of a solution with hydrogen ion concentration of 0.0001 M is ______________. Answer: *4* FBQ5: The basic distinguishing feature between peptide and protein is in respect to their ___________. Answer: *Molecular weight*   FBQ6: A _______ centre is a carbon atom to which four different functional groups are covalently linked. Answer: *Chiral* FBQ7: When an amino acid rotates the plane polarised light to the right is said to be __________ Answer: *Dextrorotatory* FBQ8: E +S↔?→E+P Complete the given equation. Answer: *ES* FBQ9: An enzyme with code [EC 1.3.2.2 ] belongs to the class of enzymes called _______ Answer: *Oxidoreductases* FBQ10: A complete catalytically active enzyme together with its bound co-enzyme and/or metal ion is called _______ Answer: *holoenzyme* FBQ11: ________ are biopolymers of amino acids in which amino acids are joined by peptide bonds. Answer: *Peptides* FBQ12: The presence of a red colour with Millon’s Reagent indicates the presence of the amino acid _______ Answer: *tryptophan* FBQ13: The enzyme with code. [EC 3.2.1.18] belongs to the class _________ Answer: *hydrolases* FBQ14: The part of an amino acid that gives it its unique property is the ___________ Answer: *side chain* FBQ15: The pH of a solution with 0.82 M hydrogen ion concentration is ______________. Answer: *13.9* FBQ16: Calculate the pH of a solution with hydrogen ion concentration of 0.00456 M to the nearest whole number __________ Answer: *2* FBQ17: Give the name of the amino acid with the given structure_________ Answer: *Alanine* FBQ18: Calculate the pH of a buffer solution which is 0.05 M in sodium acetate and 0.1 M in acetic acid. The pKa for acetic acid is 4.73 _______  Answer: *4.43* FBQ19: The α- carbon in the amino acid lysine can also be referred to as in Arabic numeral as carbon atom ______ Answer: *2* FBQ20: How many peptide residues are present in Glutathione? Answer: *Three* FBQ21: A solution that resists slight changes in pH when little amount of acid or base is added to it is known as __________ Answer: *Buffer solution* FBQ22: In a linear peptide polymer, the end of the linear polymer with free α- amino group is known as _____- Answer: *N *terminal FBQ23: How many amino acid residues are present in the peptide hormone oxytocin? ______ Answer: *8* FBQ24: The first protein to be sequenced was_______ Answer: *Insulin* FBQ25: Enzyme activity in the enzyme takes place an area known as _________ Answer: *Active site* FBQ26: A widely used sequencing procedure that identifies amino acids singly, beginning with the N-terminal residues inwards is known as___________ Answer: *Edman Degradation* FBQ27: Name the amino acid that gives a red coloured compound in Sakaguchi Reaction ________ Answer: *Arginine* FBQ28: Name the amino acid represented by the given structure_________ Answer: *Cysteine* FBQ29: Name the amino acid represented by the given structure________ Answer: *Phenylalanine* FBQ30: In the formation of peptides, amino acids are linked together in a covalent bond known as ______ Answer: *Amide linkage* FBQ31: In Gel filtration protein molecules are separated based on their shapes and _____ Answer: *size*s FBQ32: The side chain in alanine is replaced with __________. Answer: *Methyl group* FBQ33: __________ are the building blocks of proteins. Answer: *Amino acids* FBQ34: The pigment responsible for coloration in the skin is _____ Answer: *Melanin* FBQ35: How many functional groups are typical of an amino acid has __________. Answer: *Four* Multiple Choice Questions (MCQs): MCQ1: One of these amino acid has an isopropyl R group _______ Answer: Alanine MCQ2: When phenylalanine is available in the body, which of these amino acids can readily be formed? Answer: Tyrosine MCQ3: Lack of one of these amino acids in the diet will affect the synthesis of a new protein Answer: Glutamate MCQ4: Which of these amino acids is present in a test sample that gives a red colouration with Millions reagent? Answer: Tryptophan MCQ5: Which of these amino acids has an aliphatic side chain terminating with a basic group Answer: Leucine MCQ6: Which of the following is not true of the buffer action? Answer: If acid is added, the conjugate base interacts with it to give water and so resist the change in the pH. MCQ7: Calculate the pKa of lactic acid given that at pH 4.8, the concentration of lactic acid and its conjugate base is 0.001 and 0.087 M respectively. Answer: 2.86 MCQ8: One of these statements is NOT true of amino acids Answer: The R group of each amino acid is covalently linked to the carboxyl group MCQ9: Which of these amino acid will absorb light most strongly in the ultraviolet region(λ=100 to 400 nm) Answer: Phenylalanine MCQ10: One of these amino acids will give a red coloration in Sakaguchi reaction Answer: Lysine MCQ11: For an amino acid to be an alpha amino acid, which of these must be true? Answer: The amino group and the carboxyl group must be attached to the same carbon atom MCQ12: Which of the following may be formed when free α-amino groups of amino group react with aldehydes? Answer: Schiff base MCQ13: Amino acids are considered amphoteric for one of the following reasons Answer: They have both basic and acidic properties MCQ14: Which of these amino acids contain sulphur? Answer: Methionine MCQ15: One of these amino acids has an OH side chain Answer: serine MCQ16: Which of these is not a criterion for the classification of common amino acids?  Answer: Polarity of the side chain MCQ17: Which of these amino acid will not rotate the plane of a plane-polarised light? Answer: glutamine MCQ18: Which of these can be achieved with Xanthoproteic Reactions of amino acids? Answer: Differentiating between Aromatic and Non aromatic amino acids MCQ19: Which of these amino acids does NOT give a purple colour with Ninhydrin? Answer: Proline MCQ20: The bluish- purple compound appearing as ring at the interface in the reaction of tryptophan with formaldehyde in the presence of Sulphuric acid is due to the presence of________ Answer: Indole ring MCQ21: How many stereoisomers of the amino acid, Alanine, are possible given that number of stereoisomers is 2n? Where n is the number of chiral carbons present. Answer: 2 MCQ22: Which of these is not a part of the Henderson-Hasselbalch equation? Answer: Concentrated acid MCQ23: Which of these proteins is a structural protein found in hair of animals? Answer: Actin MCQ24: Which of these proteins is involved with vision? Answer: Rhodopsin MCQ25: One of these statements is NOT of proteins Answer: They are formed by a linkage between an α-amino group of one amino acid and the α-amino group of another acid MCQ26: All but one of these are types of gel materials used in gel filtration : Answer: Polyacrylamide MCQ27: Which of these methods can be used to separate protein based on their Molecular size? Answer: Ultracentrifugation MCQ28: Which of these phenomena is employed in dialysis to separate macromolecules from solvents with the aid of semi permeable membranes like cellophane Answer: diffusion MCQ29: What protein is found in Plastocyanin? Answer: zinc MCQ30: Which of these proteins is involved in motion? Answer: Collagen MCQ31: Enzymes have the following characteristic EXCEPT Answer: They cannot be deactivated MCQ32: Which of these functional groups gives each amino acid its identity Answer: The COOH group MCQ33: Which of these amino acids is an intermediate in urea formation? Answer: Ornithine MCQ34: Which of these amino acids is a hormone? Answer: Citrulline MCQ35: One of these separation techniques employs the differences in pH in separation proteins Answer: isoelectric focusing